Description

Rapid detection and quantification kit for L. monocytogenes, Salmonella spp. and E. coli producers of Shiga toxin (STEC), which allows us to obtain certifications of compliance with the sanitary standard with high precision, fast timing (only hours) and low costs.

Its use is versatile, since it has been designed to detect and quantify the three pathogens simultaneously or individually in samples of fresh salmon. Furthermore, it is capable of detecting and quantifying pathogens on processing surfaces, which allows microbiological control studies to be carried out in the production chain in order to identify sources of risk of contamination, quickly, simply and with high confidence.

The kit uses standard qPCR equipment, commonly used in molecular analysis laboratories, so it does not require investment of specific equipment. It is easy to implement and apply, at low cost and. It includes an internal control that validates the entire pathogen extraction process from the sample to detection by qPCR.

Development State

This technology has been developed to detect and quantify L. monocytogenes, Salmonella spp. and STEC in samples of fresh salmon and samples of process surfaces. Currently, it is in the validation stage, that is, to compare the results obtained with the kit and those obtained with the reference method (traditional cultivation) using real samples obtained from process plants both surface and salmon fillets.

Applications

  • Quality assurance of final product and intermediate products (fresh salmon and processing or elaboration surfaces)
  • Verification of the surface cleaning protocol.
  • Certification and risk assurance programs in plants

Opportunity

L. monocytogenes, Salmonella spp. and E. coli produce Shiga toxin (STEC) which is part of the main bacterial agents that cause foodborne illness in the world. Responsible for thousands of hospitalizations, deaths, lost work days and even plants closings and product recalls.

The food industry around the world requires analysis methods and tools that ensure food safety offered to the market with greater certainty and speed.

Until now, traditional microbiological analysis methods (plate cultures) are used and new molecular methods are being developed. The former one is slow (results in 3 to 7 days) and imprecise and the latter deliver results in less than a day and with greater confidence.

Awards

  • Copec-UC Foundation
  • Natufeed
  • Corfo